Theatrical release poster
|Directed by||Joss Whedon|
|Produced by||Barry Mendel|
|Written by||Joss Whedon|
|Music by||David Newman|
|Edited by||Lisa Lassek|
|Distributed by||Universal Pictures|
|Box office||$38.9–40.1 million|
- Nathan Fillion as Malcolm "Mal" Reynolds, a former sergeant (now captain of his privately owned ship) on the losing side of the Unification War, he struggles to survive free and independent of the Alliance.
- Gina Torres as Zoe Washburne (née Alleyne): A former corporal who fought under Mal in the war, and Wash's wife. She is the second-in command of Serenity, and is fiercely loyal to Mal, whom she addresses as "sir".
- Alan Tudyk as Hoban "Wash" Washburne: The pilot of the ship, and Zoe's husband. He often acts as a voice of reason on the ship.
- Morena Baccarin as Inara Serra, a Companion who formerly rented one of Serenity's shuttles. In one of the Operative's traps, Mal is reunited with Inara at her training house, and the two escape back to Serenity.
- Adam Baldwin as Jayne Cobb, a mercenary skilled with weapons, Jayne is often the "main gun" for jobs and is someone who can be depended on in a fight. Jayne acts and seems dumb most of the time, but may be smarter than he lets on. As Whedon states several times, he is the person that will ask the questions that no one else wants to.
- Jewel Staite as Kaywinnet Lee "Kaylee" Frye: the ship's mechanic, has an intuitive, almost symbiotic, relationship with machines and is, consequently, something of a mechanical wizard. She is also notable for a persistently bright and sunny disposition, and her crush on Simon Tam.
- Sean Maher as Simon Tam, River's loving older brother who helped rescue her from the Alliance. He and River are taken in by the crew of Serenity. A trauma surgeon before the rescue, he serves as a doctor to the crew. His life is defined by his sister's needs.
- Summer Glau as River Tam, a 17-year-old psychic genius. She and her brother are taken in by the crew of Serenity after he rescues her from an Alliance Academy where she was subjected to medical experimentation and brainwashing. Foy suggests that the Alliance "...prepares her to become an assassin and spy..." The Alliance's pursuit of River acts as the film's motive. More abstractly the film is the "story of Mal as told by River".
- Ron Glass as Shepherd Derrial Book, a shepherd, or preacher, with a mysterious past, Book was once a passenger on Serenity, but now resides on the planet Haven. Mal and the crew look to him for help.
- Chiwetel Ejiofor as The Operative, a ruthless intelligence agent of the Alliance assigned to track down River and Simon. Although Ejiofor was on the top of the casting director's list for the role, the studio wanted someone better known. Whedon was eventually able to cast Ejiofor.
- David Krumholtz as Mr. Universe, a "techno-geek" with good relations with the crew of Serenity, especially Wash, Mr. Universe lives with his "love-bot" wife and monitors incoming signals from around the universe.